PE-22-28: doses reported in published research

Sources checked 2026-10-07 · 2 cited studies (0 human, 2 animal, 0 cell culture)

PE-22-28 (also called mini-spadin) is a 7-amino-acid analog of spadin, a peptide derived from the sortilin propeptide, studied as a TREK-1 channel blocker. All published dose data come from two mouse studies by the same research group.

No human data. no human studies or registered human trials were found.

Animal studies (2)

Animal doses are listed exactly as the studies report them. They are not converted to human amounts here; doses do not translate between species by body weight.

Animal Mice (behavioural tests); PE 22-28 and its derivatives

Dose as reported: 3.0-4.0 µg/kg (single); 3.0 µg/kg (sub-chronic); 1 mg/kg by gavage (sub-chronic)
Route: Intraperitoneal; or oral gavage
Frequency / duration: Single dose 30 min before testing, or repeated (sub-chronic)

Source wording: After an acute ip administration of 3.0–4.0 μg/kg spadin-analogs Whether administered by ip injections (3.0 μg/kg) or gavage (1 mg/kg) Immobility times were measured 30 min after ip injection

Djillani A, Pietri M, Moreno S et al.. Shortened Spadin Analogs Display Better TREK-1 Inhibition, In Vivo Stability and Antidepressant Activity. Front Pharmacol 2017. PubMed PMID 28955242 ↗ · full text PMC5601071 ↗

Animal Mice (focal brain ischaemia model)

Dose as reported: 0.03 µg/kg (first 7 days); then 3 µg/kg
Route: Intraperitoneal
Frequency / duration: Once a day for 7 days from 30 min after onset; then once a day, 4 days/week

Source wording: a single low dose of mini-spadin (0.03 μg/kg) was intraperitoneally injected 30 min after the onset of ischemia, once a day during 7 days post-ischemia. the peptide was injected at higher concentration (3 μg/kg) once a day for 4 days/week until the sacrifice of animals.

Pietri M, Djillani A, Mazella J et al.. First evidence of protective effects on stroke recovery and post-stroke depression induced by sortilin-derived peptides. Neuropharmacology 2019. PubMed PMID 31325429 ↗

Half-life

No half-life value was found in the sources reviewed for this page.

About this page

Every figure above is copied from the cited source; the source wording is shown with each entry. Where a source does not state something, the page says so.
Only abstracts, open-access full texts, registry records and FDA labels were used, so details found only in a paywalled full text may be missing.
This page does not convert doses between species or into other measures, and gives no preparation or handling information.